WebNov 18, 1994 · A 4-kb fragment, containing the SIS/PDGF-B promoter, was isolated from a human genomic library, and a series of 5‘-nested deletions and linker-scanning mutants …
Molecular Cloning - an overview ScienceDirect Topics
The promoters of the selected candidate genes were identified using the available citrus genome sequences (http://citrus.pw.usda.gov/, http://www.phytozome.net/citrus.php, www.citrusgenomedb.org/) and approximately 1–3 kilobase pair region upstream of the translation initiation … See more Public citrus gene expression data available from the Gene Expression Omnibus (GEO) data repository (www.ncbi.nlm.nih.gov/geo) … See more A total of 15–20 independent transgenic tomato lines were generated for each promoter construct using Agrobacterium-mediated transformation and were grown in a greenhouse (See … See more Qualitative analysis of promoter activity was conducted by comparing the intensity of GUSPlus histochemical staining between promoter transgenic lines and controls. Images showing GUSPlus staining in various … See more WebJul 31, 2024 · These results suggest that the LmSAP promoter can be a convenient tool for stress-inducible gene expression and is a potential candidate for crop genetic engineering. ... Zhang W, Cao J, Wu R. Isolation of an efficient actin promoter for use in rice transformation. Plant Cell. 1990;2(2):163–71. pmid:2136633 . View Article PubMed/NCBI Google ... mass tort litigation process
Isolation of novel citrus and plum fruit promoters and their functional
WebOct 12, 2013 · For the functional characterization of gene expression, isolation and structural analysis of the region upstream of a gene is required. After the promoter region is isolated, the regions that activate (i.e., enhancers) or repress (i.e., silencers) gene expression are identified by deleting the promoter region [ 4 ]. WebJun 30, 2009 · Promoter is a specific DNA sequence involved in the transcription of a particular gene. It is usually located in the upstream of the gene they regulate. Isolation and characterization of... WebIsolation of the promoter of Psp68. Pea genomic DNA library was prepared by digesting genomic DNA with different restriction enzymes (EcoRV, DraI, PvuII and SspI) in 4 separate tubes to generate blunt ends of genomic DNA. The digested genomic DNA was purified and further ligated into BD genome walker kit. The primary PCR was done by using AP1 ... hygiene 2 health