Fast sampling of the cellular metabolome

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WebHere, we summarize a protocol for metabolome analysis of yeast cells using gas chromatography-mass spectrometry (GC-MS). First, the main phases of a metabolomics … WebObtaining meaningful snapshots of the metabolome of microorganisms requires rapid sampling and immediate quenching of all metabolic activity, to prevent any changes in …

Automated fast filtration and on-filter quenching improve the ...

WebFast sampling of the cellular metabolome — TU Delft Research Portal Researchers Research output Datasets Projects Fast sampling of the cellular metabolome WM van … WebSep 1, 2006 · A sampling frequency of 5 s(-)(1) and an overall individual sample processing time faster than 30 s allow observing responses of intracellular metabolite concentrations to extracellular stimuli on a subsecond time scale. Recovery and reliability of the unit operations were analyzed. lampenmeister shop

Cell Metabolomics - PMC - National Center for Biotechnology …

WebFast sampling systems are currently characterized by motorized sampling, high frequency, negligible dead areas, and efficient inactivation [69][70][71]. The technology developed by van Gulik... WebMar 1, 2014 · Our results suggested that the fast filtration sampling method with a vacuum manifold system can be widely applied to yeast metabolome analysis due to its simplicity, robustness, and lack of ... WebJan 1, 2012 · This automated rapid sampling device was applied for investigation of the intracellular metabolite dynamics of glycolysis in E. … lampe michel boyer

Fast filtration for metabolome sampling of suspended …

Fast Sampling of the Cellular Metabolome - PubMed

The metabolome refers to the complete set of small-molecule chemicals found within a biological sample. The biological sample can be a cell, a cellular organelle, an organ, a tissue, a tissue extract, a biofluid or an entire organism. The small molecule chemicals found in a given metabolome may include both endogenous metabolites that are naturally produced by an organism (such as a… WebDetailed protocols are provided for rapid sampling, quenching, and extraction, for measurement of metabolites in total broth samples, washed cell samples, and … lampenkraut genshin impactWebThe protocol developed involves rapid collection by spraying the sample into -40 degrees C tricine-buffered methanol (as previously described for yeast metabolome analysis), … help for women

"WebJan 1, 2012 · Detailed protocols are provided for rapid sampling, quenching, and extraction for measurement of metabolites in total broth samples, washed cell samples and … " - Fast sampling of the cellular metabolome

Fast sampling of the cellular metabolome

Yeast Metabolomics: Sample Preparation for a GC/MS-Based …

WebA new method for sampling suspended animal cells by fast filtration is presented that allows rapid quenching of cellular metabolism and efficient separation of the cells from … WebFast Sampling of the Cellular Metabolome Walter M. van Gulik, Andre B. Canelas, Hilal Taymaz-Nikerel, Rutger D. Douma, Lodewijk P. de Jonge, and Joseph J. Heijnen Abstract Obtaining meaningful snapshots of the metabolome of microorganisms requires rapid …

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WebObtaining meaningful snapshots of the metabolome of microorganisms requires rapid sampling and immediate quenching of all metabolic activity, to prevent any changes in …

WebA simple, fast, and reproducible sample preparation procedure has been developed for relative quantification of metabolites in adherent mammalian cells using the clonal β-cell … WebAug 1, 2024 · For metabolome sample preparation, rapid quenching of cellular metabolism without severe loss of intracellular metabolites is important. Here, we comparatively evaluated the two sampling...

WebMay 1, 2007 · Sampling for intracellular amino acids was performed via fast filtration and subsequent extraction in boiling water as previously validated and applied for such analysis in C. glutamicum (Bolten ... WebMetabolome sample preparation is one of the key factors in metabolomics analyses. The quality of the metabolome data will depend on the suitability of the experimental procedures to the cellular system (e.g., yeast cells) and the analytical performance.

WebApr 20, 2012 · Fast Sampling of the Cellular Metabolome DOI: 10.1007/978-1-61779-827-6_10 Source Authors: Walter van Gulik Delft University of Technology André Canelas …

WebDetailed protocols are provided for rapid sampling, quenching, and extraction, for measurement of metabolites in total broth samples, washed cell samples, and … help for women\u0027s abuseWebApr 14, 2024 · An overshoot of H 2 O 2 impairs cell function through oxidative damage of proteins, thereby, inducing cellular dysfunction or death 2. The redox-modified molecules are mostly affected... lampen online poldinaWebFast filtration for metabolome sampling of suspended animal cells . Epub 2010 Nov 12. Authors Martin Volmer 1 , Stefan Northoff , Sebastian Scholz , Tobias Thüte , Heino Büntemeyer , Thomas Noll Affiliation 1 Institute of Cell Culture Technology, Bielefeld University, Bielefeld, Germany. [email protected] PMID: 21072561 help for women starting overWebJan 1, 2024 · Detailed protocols are provided for rapid sampling, quenching, and extraction, for measurement of metabolites in total broth samples, washed cell samples, and supernatant, to be applied for quantitative metabolomics of both eukaryotic and prokaryotic microorganisms. Full text links Read article at publisher's site (DOI): help for women abusedWebSep 1, 2009 · Approximately 2 mg of biomass was quenched into 100% methanol, which was chilled at -40°C prior, using a rapid sampling setup [30]. Sample filtrate was washed twice with 20 mL 80% (v/v)... help for women of domestic abuseWebNov 1, 2010 · The current major bottleneck for wide spread use of metabolomics and delivery of highly reproducible and reliable results is the sampling of suspension cultured cells while maintaining the levels... help for women in crisisWebOne of the most common workflows to characterize complex proteomes is based on a bottom-up MS approach, which aims to quantitatively extract and digest proteins chemically and enzymatically from isolated cells or tissues, generate peptides and deliver them to a ready-to-analyze separation platform in sufficient quantities to enable a robust … help for women in abusive marriage