Chromium next gem chip g single cell kit
WebThe 10x Genomics Chromium Controller is a single-cell profiling technology that enables the analysis of large cell numbers at a high capture efficiency (of up to 65%). The … Web1 day ago · Single-cell complementary DNA (cDNA) libraries were prepared using the Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 according to the manufacturer’s instructions. Samples were run using 10x Genomics based on manufacturer’s protocol, capturing 8,600 to 22,000 cells for each sample.
Chromium next gem chip g single cell kit
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WebNov 18, 2024 · Note: Next GEM (Gel Bead-In EMulsions) is a technology that is built on a new chip architecture, the Next GEM Single Cell 3′ Reagent Kits v3.1 and Next GEM Chip G Single Cell Kits allow for a high number of detected genes, minimizes the number of barcodes that can be interpreted to come from more than one cell, 65% capture rate, … WebSingle cells were processed using the Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1 and Chromium Next GEM Chip G Single Cell Kit according to the manufacturer’s instructions in the Chromium Next GEM Single Cell 3′ Reagents Kits V3.1 User Guide(10X Genomics). Briefly, 6,000 cells were loaded into each chip channel ...
WebMicrobiome Research Laboratory (MRL) loacted at Biocenter of UT Southwestern Medical Center provides next generation sequencing services to basic and translational researchers who wish to use microbial metagenomics in their research activities. WebChromium Next GEM Automated Single Cell 3’ Library and Gel Bead Kit v3.1, 4 rxns PN-1000147 Chromium Next GEM Automated Chip G Single Cell Kit, 48 rxns PN-1000136 Chromium Next GEM Automated Chip G Single Cell Kit, 16 rxns PN-1000146 Single Index Kit T Set A, 96 rxns PN-1000213 Dual Index Kit TT Set A, 96 rxns PN-1000215
WebChromium Next GEM Automated Single Cell 3’ Library and Gel Bead Kit v3.1 4 rxns: Product code 1000147 24 rxns: Product code 1000141 Chromium Next GEM Chip G Automated Single Cell Kit 16 rxns: Product code 1000146 48 rxns: Product code 1000136 Dual Index Kit TT Set A 96 rxns: Product code 1000215 Chromium Next GEM …
WebChromium Next GEM Automated Single Cell 3’ Reagent Kits v3.1: Chromium Next GEM Chip G Automated Single Cell Kit, 48 rxns PN-1000136 Chromium Next GEM Chip G Automated Single Cell Kit, 16 rxns PN-1000146: 50% Glycerol 2000109 ! " # $ # # % & ' … crystal hollingerWebApr 1, 2024 · The cell suspension for each pool was prepared with the Chromium Next GEM Single Cell 5' v2 and Chromium Next GEM Chip K Single-Cell Kit and loaded into Chromium Controller equipment. Once the single cells were obtained by the formation of gel bead emulsions (GEMs), libraries were prepared using a Chromium Single Cell … dwhelp redditWebApr 28, 2024 · snRNA-seq targeting 9,000 cells per sample was performed using Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 (10x Genomics, 1000121) and Chromium Next GEM Chip G Single Cell Kit (10x Genomics, 1000120). Library strategy: RNA-Seq: Library source: transcriptomic single cell: Library selection: cDNA: Instrument … crystal holidays manage my bookingWebassets.ctfassets.net dwhelper reviewWebFeb 9, 2024 · Chromium Next Gem Single Cell 3′ GEM, Library & Gel Bead Kit v.3.1, 16rnx: 10X Genomics: Cat# PN-1000121: Chromium Next GEM Chip G Single Cell Kit, 16 rxns: 10X Genomics: Cat# PN-1000127: Single Index Kit T Set A, 96 rnx: 10X Genomics: Cat# PN-1000213: Buffer EB: Qiagen: Cat# 19086: Qubit 4.0 Fluorometer: Thermo … dwhelper shortcutWebJul 12, 2024 · Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Single Index Kit T Set A, 96 rxns PN-1000213 Important Upgrading to Firmware 4.00 is … dwhelper resume downloadWebJun 18, 2024 · Add the following volumes of serum/LIF culture media and gently pipet up and down to create a single-cell suspension: i. 2.5 mL per 6-well. ii. 9 mL per 100mm plate. iii. 15 mL per 150mm plate. f. Split cells at a ratio of 1:5 to 1:8 into a new gelatinized tissue-culture plate with pre-warmed media. g. crystal holliday bird dog traffic control